Journal: Scientific Reports

Article Title: Evaluation of commercially available fully automated and ELISA-based assays for detecting anti-SARS-CoV-2 neutralizing antibodies

doi: 10.1038/s41598-022-21317-x

Figure Lengend Snippet: Analytical performance of the evaluated sVNT assays using the pre-defined cutoff values and the newly calculated cutoff values determined using Youden's index.

Article Snippet: Further, GenScript cPass sVNT exhibited the best linear correlation with pVNT in samples collected from both infected and vaccinated individuals.

Techniques: Infection

Journal: Heliyon

Article Title: Performance and correlation of ten commercial immunoassays for the detection of SARS-CoV-2 antibodies

doi: 10.1016/j.heliyon.2022.e12614

Figure Lengend Snippet: Details of the evaluated ten commercial immunoassays.

Article Snippet: All assays showed moderate to high correlation with GenScript sVNT (r = 0.58 to r = 0.98), except Roche total antibodies (r = 0.48).

Techniques: Chemiluminescence Immunoassay, Enzyme-linked Immunosorbent Assay, Inhibition, Virus, Neutralization

Journal: Heliyon

Article Title: Performance and correlation of ten commercial immunoassays for the detection of SARS-CoV-2 antibodies

doi: 10.1016/j.heliyon.2022.e12614

Figure Lengend Snippet: The sensitivity and specificity of each commercial immunoassay.

Article Snippet: All assays showed moderate to high correlation with GenScript sVNT (r = 0.58 to r = 0.98), except Roche total antibodies (r = 0.48).

Techniques:

Journal: Heliyon

Article Title: Performance and correlation of ten commercial immunoassays for the detection of SARS-CoV-2 antibodies

doi: 10.1016/j.heliyon.2022.e12614

Figure Lengend Snippet: Lists of false positives, identified acute infecting pathogens, and results from all assays.

Article Snippet: All assays showed moderate to high correlation with GenScript sVNT (r = 0.58 to r = 0.98), except Roche total antibodies (r = 0.48).

Techniques:

Journal: Heliyon

Article Title: Performance and correlation of ten commercial immunoassays for the detection of SARS-CoV-2 antibodies

doi: 10.1016/j.heliyon.2022.e12614

Figure Lengend Snippet: Violin Plot of nine COVID-19 commercial immunoassays for detecting SARS-CoV-2 antibodies compared to GenScript sVNT. (A). Abbott IgG compared to GenScript sVNT. (B). FREND™ IgG compared to GenScript sVNT. (C). Roche total antibodies compared to GenScript sVNT. (D). Snibe IgG compared to GenScript sVNT. (E). Beckman IgG compared to GenScript sVNT. (F). Mindray IgG compared to GenScript sVNT. (G). Mindray total antibodies compared to GenScript sVNT. (H). Siemens total antibodies compared to GenScript sVNT. (I). Vazyme sVNT compared to GenScript sVNT.

Article Snippet: All assays showed moderate to high correlation with GenScript sVNT (r = 0.58 to r = 0.98), except Roche total antibodies (r = 0.48).

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Analytical performance of each kit in discriminating SARS-CoV-2 infection.

Article Snippet: For the GenScript cPass sVNT kit, dilution was not conducted because the method represents the result of the inhibition ratio calculated from the optical density measured, which does not necessarily linearly correlate with the titer of the antibodies.

Techniques: Infection

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Analytical performance for representativeness of neutralizing activity using the pre-defined cut-off values of each immunoassay kit.

Article Snippet: For the GenScript cPass sVNT kit, dilution was not conducted because the method represents the result of the inhibition ratio calculated from the optical density measured, which does not necessarily linearly correlate with the titer of the antibodies.

Techniques: Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Titer correlation of the analytical performance of the prediction of neutralizing activity using newly calculated cut-off values determined using Youden’s index.

Article Snippet: For the GenScript cPass sVNT kit, dilution was not conducted because the method represents the result of the inhibition ratio calculated from the optical density measured, which does not necessarily linearly correlate with the titer of the antibodies.

Techniques: Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Serial kinetics of antibody titers measured with each method: (A) PRNT ND50, (B.) GenScript cPass sVNT, (C) Roche Elecsys Anti-SARS-CoV-2, (D) Roche Elecsys Anti-SARS-CoV2 S, and (E) Abbott AdviseDx SARS-CoV2 IgG II.

Article Snippet: For the GenScript cPass sVNT kit, dilution was not conducted because the method represents the result of the inhibition ratio calculated from the optical density measured, which does not necessarily linearly correlate with the titer of the antibodies.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Antibody titers by timeline.

Article Snippet: For the GenScript cPass sVNT kit, dilution was not conducted because the method represents the result of the inhibition ratio calculated from the optical density measured, which does not necessarily linearly correlate with the titer of the antibodies.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Scatter plot and Pearson’s correlation for each method grouped with acute/convalescent phase. (A) GenScript cPass sVNT, (B) Roche Elecsys Anti-SARS-CoV-2, (C) Roche Elecsys Anti-SARS-CoV2 S, and (D) Abbott AdviseDx SARS-CoV2 IgG II were compared with PRNT, respectively. Each colored line depicts the linear regression model and the surrounding grey-colored area represents the 95% confidence interval.

Article Snippet: For the GenScript cPass sVNT kit, dilution was not conducted because the method represents the result of the inhibition ratio calculated from the optical density measured, which does not necessarily linearly correlate with the titer of the antibodies.

Techniques: